详细信息

果糖标准品 fructose standard 500 mg
葡萄糖检测试剂 glucose assay reagent 1 小瓶
磷酸葡萄糖异构酶 phosphoglucose isomerase 1 小瓶

Description
Application This kit is for the quantitative, enzymatic determination of fructose in food and other materials.Fructose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Fructose 6-phosphate is converted to glucose 6-phosphate by phosphoglucose isomerase (PGI). Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in the reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to fructose concentration.



Properties
usage sufficient for 20 assays
storage temp. 2-8°C



References
reference Beuter, H.O. Meth. Enzym. Anal. 3rd ed., 6, 321, (1985)
Official Methods of Analysis of the AOAC 16th ed., Arlington, VA , (1995), 28.1.17

Printable Reviews

Components
Standard component